{"id":31,"date":"2021-10-28T08:15:03","date_gmt":"2021-10-28T08:15:03","guid":{"rendered":"http:\/\/cn.celetrix.com\/celetrix\/?page_id=31"},"modified":"2021-11-07T10:17:35","modified_gmt":"2021-11-07T10:17:35","slug":"genome-editing","status":"publish","type":"page","link":"https:\/\/cn.celetrix.com\/zh\/applications\/genome-editing\/","title":{"rendered":"\u57fa\u56e0\u7f16\u8f91"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"31\" class=\"elementor elementor-31\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2cd4bcc0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2cd4bcc0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-42465f82\" data-id=\"42465f82\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4d81b117 elementor-widget elementor-widget-bdt-breadcrumbs\" data-id=\"4d81b117\" data-element_type=\"widget\" data-widget_type=\"bdt-breadcrumbs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class='bdt-breadcrumbs-wrapper'><ul class=\"bdt-breadcrumb breadcrumb-align-left\"><li><a href=\"https:\/\/cn.celetrix.com\/zh\/\"><span class=\"bdt-breadcrumbs-home-icon\"> <i aria-hidden=\"true\" class=\"fas fa-home\"><\/i> <\/span>\u5168\u65b0\u9ad8\u6548\u7ec6\u80de\u7535\u8f6c\u4eea<\/a><\/li>\n<li><span> \u9875\u9762<\/span><\/li>\n<\/ul><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1c0c6686 elementor-widget elementor-widget-heading\" data-id=\"1c0c6686\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Celetrix\u7535\u8f6c\u4eea\u5728\u57fa\u56e0\u7f16\u8f91\u9886\u57df\u7684\u5e94\u7528<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6bf01427 elementor-widget elementor-widget-text-editor\" data-id=\"6bf01427\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><img fetchpriority=\"high\" decoding=\"async\" class=\"wp-image-314 alignleft\" src=\"http:\/\/celetrix.hy-yun.com\/wp-content\/uploads\/2021\/11\/20180328103215_730.jpg\" alt=\"\" width=\"804\" height=\"256\" srcset=\"https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/20180328103215_730.jpg 597w, https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/20180328103215_730-300x95.jpg 300w\" sizes=\"(max-width: 804px) 100vw, 804px\" \/><\/p><div>\u5229\u7528Celetrix\u7684\u7535\u8f6c\u6280\u672f\u5728Human iPSC\u4e2d\u7535\u8f6cCas9\u86cb\u767d\u548cgRNA (+) \u6216\u8005 Cas9\u86cb\u767d\uff0c\u901a\u8fc7T7E1\u6838\u9178\u5185\u5207\u9176\u68c0\u6d4b\u57fa\u56e0\u7f16\u8f91\u6548\u7387\uff0c\u5728APP\uff0cAAVS1\uff0cOCT4\u548cPDCD1\u56db\u4e2a\u57fa\u56e0\u4f4d\u70b9\u7684\u7f16\u8f91\u6548\u7387\u5747\u8d85\u8fc750%\uff0c\u5176\u4e2dAAVS1\u57fa\u56e0\u4f4d\u70b9\u7f16\u8f91\u6548\u7387\u80fd\u591f\u8fbe\u523070%\u3002<\/div><p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9d38767 elementor-widget elementor-widget-text-editor\" data-id=\"9d38767\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><img decoding=\"async\" class=\"alignnone size-full wp-image-511\" src=\"http:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/gRNA_2.png\" alt=\"\" width=\"597\" height=\"192\" srcset=\"https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/gRNA_2.png 597w, https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/gRNA_2-300x96.png 300w, https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/gRNA_2-18x6.png 18w\" sizes=\"(max-width: 597px) 100vw, 597px\" \/> <img decoding=\"async\" class=\"alignnone size-full wp-image-512\" src=\"http:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/gRNA_3.jpg\" alt=\"\" width=\"597\" height=\"189\" srcset=\"https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/gRNA_3.jpg 597w, https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/gRNA_3-300x95.jpg 300w, https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/gRNA_3-18x6.jpg 18w\" sizes=\"(max-width: 597px) 100vw, 597px\" \/><\/p><p>\u5229\u7528Celetrix\u7684\u7535\u8f6c\u6280\u672f\u7535\u8f6cCas9\u86cb\u767d\u548cgRNA (+) \u6216\u8005 Cas9\u86cb\u767d\uff0c\u901a\u8fc7T7E1\u6838\u9178\u5185\u5207\u9176\u68c0\u6d4b\u57fa\u56e0\u7f16\u8f91\u6548\u7387\uff0c\u65e0\u8bba\u662f\u5e38\u89c1\u7684HEK293\u7ec6\u80de\uff0c\u8fd8\u662f\u8f83\u96be\u7535\u8f6c\u7684Human iPSC\u548cHuman T\u7ec6\u80de\uff0c\u5728AAVS1\uff0cOCT4\u548cPDCD1\u4e09\u4e2a\u57fa\u56e0\u4f4d\u70b9\u7684\u7f16\u8f91\u6548\u7387\u5747\u8d85\u8fc750%\uff0c\u5176\u4e2dAAVS1\u57fa\u56e0\u4f4d\u70b9\u7f16\u8f91\u6548\u7387\u80fd\u591f\u8fbe\u523070%\u4ee5\u4e0a\u3002<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8aa837c elementor-widget elementor-widget-text-editor\" data-id=\"8aa837c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-313\" src=\"http:\/\/celetrix.hy-yun.com\/wp-content\/uploads\/2021\/11\/20180308170627_938.png\" alt=\"\" width=\"1237\" height=\"219\" srcset=\"https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/20180308170627_938.png 1237w, https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/20180308170627_938-300x53.png 300w, https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/20180308170627_938-1024x181.png 1024w, https:\/\/cn.celetrix.com\/wp-content\/uploads\/2021\/11\/20180308170627_938-768x136.png 768w\" sizes=\"(max-width: 1237px) 100vw, 1237px\" \/><div>\u5229\u7528Celetrix\u7684\u7535\u8f6c\u6280\u672f\u5728\u4eba\u7c7b\u539f\u4ee3T\u7ec6\u80de\u4e2d\u6572\u9664PD-1\u57fa\u56e0\uff0c\u7535\u8f6cCas9\/gRNA RNP \u65f6\uff0c\u53ef\u4ee5\u5c06PD-1\u5728\u7ec6\u80de\u8868\u9762\u7684\u8868\u8fbe\u4ece 31.9%\u964d\u4f4e\u523018.3%\uff0c\u5f53\u540c\u65f6\u7535\u8f6c\u8fdb\u53bb\u5305\u542b\u79fb\u7801\u7a81\u53d8\u7684ssODN\u65f6\uff0c\u53ef\u4ee5\u5c06PD-1\u5728\u7ec6\u80de\u8868\u9762\u7684\u8868\u8fbe\u4e0b\u964d\u523013.2%\u3002<\/div><div><div>\u6570\u636e\u6765\u81ea\u6587\u732e\uff1a<\/div><div>Sci Rep. 2018 Aug 3;8(1):11649. doi: 10.1038\/s41598-018-30227-w.<\/div><div>Efficient homology-directed gene editing by CRISPR\/Cas9 in human stem and primary cells using tube electroporation.<\/div><div>Xu X, Gao D, Wang P, Chen J, Ruan J, Xu J, Xia X.<\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-107fb73e\" data-id=\"107fb73e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-21740019 elementor-nav-menu__align-start elementor-nav-menu--dropdown-none elementor-widget elementor-widget-nav-menu\" data-id=\"21740019\" data-element_type=\"widget\" data-settings=\"{&quot;layout&quot;:&quot;vertical&quot;,&quot;submenu_icon&quot;:{&quot;value&quot;:&quot;&lt;i class=\\&quot;fas fa-caret-down\\&quot;&gt;&lt;\\\/i&gt;&quot;,&quot;library&quot;:&quot;fa-solid&quot;}}\" data-widget_type=\"nav-menu.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<nav aria-label=\"Menu\" class=\"elementor-nav-menu--main elementor-nav-menu__container elementor-nav-menu--layout-vertical e--pointer-background e--animation-fade\">\n\t\t\t\t<ul id=\"menu-1-21740019\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-286\"><a href=\"https:\/\/cn.celetrix.com\/zh\/applications\/car-tcar-nk\/\" class=\"elementor-item\">CAR-T\u548cCAR-NK<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-287\"><a href=\"https:\/\/cn.celetrix.com\/zh\/applications\/genome-editing\/\" class=\"elementor-item\">\u57fa\u56e0\u7f16\u8f91<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-285\"><a href=\"https:\/\/cn.celetrix.com\/zh\/applications\/antibody-production\/\" class=\"elementor-item\">\u6297\u4f53\u751f\u4ea7<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-288\"><a href=\"https:\/\/cn.celetrix.com\/zh\/applications\/stem-cell-therapy\/\" class=\"elementor-item\">\u5e72\u7ec6\u80de\u7535\u8f6c<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<nav class=\"elementor-nav-menu--dropdown elementor-nav-menu__container\" aria-hidden=\"true\">\n\t\t\t\t<ul id=\"menu-2-21740019\" class=\"elementor-nav-menu sm-vertical\"><li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-286\"><a href=\"https:\/\/cn.celetrix.com\/zh\/applications\/car-tcar-nk\/\" class=\"elementor-item\" tabindex=\"-1\">CAR-T\u548cCAR-NK<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-287\"><a href=\"https:\/\/cn.celetrix.com\/zh\/applications\/genome-editing\/\" class=\"elementor-item\" tabindex=\"-1\">\u57fa\u56e0\u7f16\u8f91<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-285\"><a href=\"https:\/\/cn.celetrix.com\/zh\/applications\/antibody-production\/\" class=\"elementor-item\" tabindex=\"-1\">\u6297\u4f53\u751f\u4ea7<\/a><\/li>\n<li class=\"menu-item menu-item-type-post_type menu-item-object-page menu-item-288\"><a href=\"https:\/\/cn.celetrix.com\/zh\/applications\/stem-cell-therapy\/\" class=\"elementor-item\" tabindex=\"-1\">\u5e72\u7ec6\u80de\u7535\u8f6c<\/a><\/li>\n<\/ul>\t\t\t<\/nav>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Electroporation by Celetrix Page Celetrix\u7535\u8f6c\u4eea\u5728\u57fa\u56e0\u7f16\u8f91\u9886\u57df\u7684\u5e94\u7528 \u5229\u7528Celetrix\u7684\u7535\u8f6c\u6280\u672f\u5728Human iPSC\u4e2d\u7535\u8f6cCas9\u86cb\u767d\u548cgRNA (+) \u6216\u8005 Cas9\u86cb\u767d\uff0c\u901a\u8fc7T7E1\u6838\u9178\u5185\u5207\u9176\u68c0\u6d4b\u57fa\u56e0\u7f16\u8f91\u6548\u7387\uff0c\u5728APP\uff0cAAVS1\uff0cOCT4\u548cPDCD1\u56db\u4e2a\u57fa\u56e0\u4f4d\u70b9\u7684\u7f16\u8f91\u6548\u7387\u5747\u8d85\u8fc750%\uff0c\u5176\u4e2dAAVS1\u57fa\u56e0\u4f4d\u70b9\u7f16\u8f91\u6548\u7387\u80fd\u591f\u8fbe\u523070%\u3002 \u00a0 \u5229\u7528Celetrix\u7684\u7535\u8f6c\u6280\u672f\u7535\u8f6cCas9\u86cb\u767d\u548cgRNA (+) \u6216\u8005 Cas9\u86cb\u767d\uff0c\u901a\u8fc7T7E1\u6838\u9178\u5185\u5207\u9176\u68c0\u6d4b\u57fa\u56e0\u7f16\u8f91\u6548\u7387\uff0c\u65e0\u8bba\u662f\u5e38\u89c1\u7684HEK293\u7ec6\u80de\uff0c\u8fd8\u662f\u8f83\u96be\u7535\u8f6c\u7684Human iPSC\u548cHuman T\u7ec6\u80de\uff0c\u5728AAVS1\uff0cOCT4\u548cPDCD1\u4e09\u4e2a\u57fa\u56e0\u4f4d\u70b9\u7684\u7f16\u8f91\u6548\u7387\u5747\u8d85\u8fc750%\uff0c\u5176\u4e2dAAVS1\u57fa\u56e0\u4f4d\u70b9\u7f16\u8f91\u6548\u7387\u80fd\u591f\u8fbe\u523070%\u4ee5\u4e0a\u3002 \u5229\u7528Celetrix\u7684\u7535\u8f6c\u6280\u672f\u5728\u4eba\u7c7b\u539f\u4ee3T\u7ec6\u80de\u4e2d\u6572\u9664PD-1\u57fa\u56e0\uff0c\u7535\u8f6cCas9\/gRNA RNP \u65f6\uff0c\u53ef\u4ee5\u5c06PD-1\u5728\u7ec6\u80de\u8868\u9762\u7684\u8868\u8fbe\u4ece 31.9%\u964d\u4f4e\u523018.3%\uff0c\u5f53\u540c\u65f6\u7535\u8f6c\u8fdb\u53bb\u5305\u542b\u79fb\u7801\u7a81\u53d8\u7684ssODN\u65f6\uff0c\u53ef\u4ee5\u5c06PD-1\u5728\u7ec6\u80de\u8868\u9762\u7684\u8868\u8fbe\u4e0b\u964d\u523013.2%\u3002\u6570\u636e\u6765\u81ea\u6587\u732e\uff1aSci Rep. 2018 Aug 3;8(1):11649. doi: 10.1038\/s41598-018-30227-w.Efficient homology-directed gene editing by CRISPR\/Cas9 in human stem and primary cells using tube electroporation.Xu X, Gao D, Wang P, Chen J, Ruan J, Xu J, Xia X.<\/p>","protected":false},"author":1,"featured_media":0,"parent":11,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"class_list":["post-31","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/31","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/comments?post=31"}],"version-history":[{"count":7,"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/31\/revisions"}],"predecessor-version":[{"id":515,"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/31\/revisions\/515"}],"up":[{"embeddable":true,"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/pages\/11"}],"wp:attachment":[{"href":"https:\/\/cn.celetrix.com\/zh\/wp-json\/wp\/v2\/media?parent=31"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}